H5N1 Influenza Pseudovirus

Comprehensive Neutralization Assay Solutions for Advancing Influenza Research

Get your pseudovirus

Influenza Stain: A/Viet Nam/1194/2004 (H5N1)

We produce a pseudovirus pseudotyped exclusively with the hemagglutinin protein of the influenza strain A/Viet Nam/1194/2004(H5N1) (GenBank: EF541402.1), which was responsible for the outbreak in China in 2004.

Infectious titer of at least 10⁴ RLU/µL

The transduction efficiency is evaluated using HEK293T cells.

Stable for at least 6 months at -80°C

Long-term stability data are updated over time as it becomes available.

Resistant to 10 freeze-thaw cycles

The custom-made storage solution allows the pseudovirus to remain stable through multiple freeze-thaw cycles.

Validated in neutralization assay

Each batch of H5 pseudovirus is validated using our neutralizing antibody anti-H5N1.

Perform up to 1,000 reactions per mL

We can provide you with highly efficient H5 pseudovirus across various volume scales.

Standard neutralizing antibody available

Ensure the reliability of your results by using the standard NAbs employed to validate the H5 influenza pseudovirus.

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IVANO's Pseudovirus Advantages

Quality control performed on each batch

Data-proven neutralization

Characterization of thermal stability

Support high-throughput screening

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H5N1 Pseudovirus Features

Enhanced safety

Use of a 3rd-generation lentivirus system

Enable specific detection

Expression of hemagglutinin as the sole surface antigen

Sensitive and easy detection

Encoding for firefly luciferase

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H5N1 Pseudovirus in Publications

The increasing number of influenza cases caused by the highly pathogenic avian influenza (HPAI) H5N1 has accelerated the need to develop vaccines and antiviral agents against influenza A viruses (IAVs). The pseudovirus neutralization assay, which does not involve infectious viruses and can be performed without a biosafety level 3 laboratory, offers a safe and rapid method for screening neutralizing monoclonal antibodies (mAbs) and antiviral agents targeting the hemagglutinin (HA) of IAVs.

• Development of a safe and convenient neutralization assay for rapid screening of influenza HA-specific neutralizing monoclonal antibodies
• Highly Pathogenic Avian Influenza A(H5N1) Mutants Transmissible by Air Are Susceptible to Human and Animal Neutralizing Antibodies
• Analysis of hemagglutinin-mediated entry tropism of H5N1 avian influenza

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H5N1 Pseudovirus Applications

Pseudoviruses provide a safe and versatile platform for influenza virus research and the development of therapeutic countermeasures. Influenza pseudoviruses have several important applications in research and development, including:

Vaccine Development

They are used to test the efficacy of new vaccines by evaluating their ability to induce neutralizing immune responses without the need for live pathogenic viruses.

Neutralizing Monoclonal Antibody Screening

Pseudoviruses enable rapid and safe screening of monoclonal antibodies targeting specific strains of the influenza virus.

Antiviral Resistance Studies

They help study viral mutations and evaluate how these mutations affect sensitivity to antiviral treatments.

Fundamental Research on Viral Entry

Pseudoviruses are used to understand the mechanisms of viral entry into host cells, which is crucial for developing new therapies targeting these processes.

Treatment Efficacy Testing

They allow for the quick assessment of new antiviral agents’ effectiveness in controlled environments without the risks associated with highly pathogenic viruses.

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H5N1 Influenza Virus Background

The H5N1 influenza virus is an avian subtype of the influenza A virus, characterized by its highly pathogenic nature. Like other influenza viruses, H5N1 has an enveloped structure with a segmented, single-stranded negative-sense RNA genome. Its eight segments, ranging from 890 to 2,341 nucleotides, encode various proteins essential for viral replication and infection. The hemagglutinin (HA) protein plays a crucial role in binding to host cell sialic acid receptors, initiating entry through clathrin-mediated endocytosis. Following endosomal acidification, the virus fuses with the vesicle membrane, releasing its RNA into the nucleus for replication. Epidemiologically, H5N1 is notable for its severe outbreaks in poultry and sporadic transmission to humans, often resulting in a high mortality rate. This zoonotic potential underscores the critical need for monitoring and developing countermeasures against potential pandemics.